PROCEED AMP2 Manuale Utente

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Infinium
®
II Assay HC Two-Sample, Automated
Experienced User Card
Part # 11280811 Rev. A Page 1 of 22
Day 1 Day 2 Day 3
Pre-Amp
Post-Amp
Optional stop,
cold storage
Overnight
incubation
Make Quant
(Optional/LIMS)
Robot: 25 min/8 samples
Reagents
Lambda DNA
PicoGreen dsDNA
1X TE
Output
QNT Plate
Standard QNT Plate
Read Quant
(Optional/LIMS)
Hands-on: ~5 min/plate
Output
Electronic File with
Information about
Sample DNA
Incubate AMP2
Incubation: 20–24 hours
Output
AMP2 Plate with
Amplified DNA
Make AMP2
Robot: 20 min/8 samples
Reagents
0.1N NaOH
MP1
AMM
Output
AMP2 Plate
Make Multi AMP2
Robot:
35 min/24 samples
Reagents
0.1N NaOH
MP1
AMM
Output
1–4 AMP2 Plates
XStain BC2
Robot:
130 min/8 samples
Dry: 1 hour
Reagents
RA1
95% Formamide /
1 mM EDTA
PB1
XC1
XC2
XC3
XC4
TEM
LTM
ATM
Output
BeadChip
Image BC2
Scan: 45 min/BeadChip
Output
Image and Data Files
Precip AMP2
Robot: 20 min/8 samples
Incubation: 55 min
Dry: 60 min
Reagents
2-propanol
PA1
Output
AMP2 Plate
Fragment AMP2
Robot: 10 min/8 samples
Incubation: 60 min
Reagents
FRG
Output
AMP2 Plate
Resuspend AMP2
Robot: 5 min/plate
Incubation: 1 hour
Reagents
RA1
Output
AMP2 Plate
Hyb HC BC2
Robot: 10 min/8 samples
Incubation: 16–24 hours
Reagents
PB2
Output
BeadChip
Wash BC2
Robot: 20 min/4
BeadChips
Reagents
WB1
PB1
Output
BeadChip
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Sommario

Pagina 1 - Experienced User Card

Infinium® II Assay HC Two-Sample, AutomatedExperienced User CardPart # 11280811 Rev. A Page 1 of 22Day 1 Day 2 Day 3Pre-AmpPost-AmpOptional stop,cold

Pagina 2 - (Optional/LIMS)

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 10 of 22[ ] 18. Place the inverted, uncovere

Pagina 3

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 11 of 22Start the Robot[ ] 1. At the robot P

Pagina 4 - Read Quant

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 12 of 22Estimated TimeRobot time:• 8 samples:

Pagina 5 - Make AMP2

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 13 of 22[ ] 1. Place the Hyb Chamber gaskets

Pagina 6

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 14 of 22Set Up HC BC2 for Hyb[ ] 1. Ensure t

Pagina 7 - AMP2 Plate

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 15 of 22Estimated TimeRobot time:• 4 BeadChips

Pagina 8 - Precip AMP2

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 16 of 22StepsWash BC2[ ] 1. Attach the wire

Pagina 9

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 17 of 22[ ] a. Gently push the glass back pl

Pagina 10 - Resuspend

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 18 of 22Estimated TimeRobot time: • 2 hours an

Pagina 11

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 19 of 22[ ] Turn on the water circulator. Se

Pagina 12 - Hyb HC BC2

Infinium® II Assay HC Two-Sample, Automated (Pre-Amp)Experienced User CardPart # 11280811 Rev. A Page 2 of 22Estimated TimeRobot time:• 20 minutes pe

Pagina 13

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 20 of 22[ ] 2. When prompted, wait for the C

Pagina 14

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 21 of 22[ ] 7. Move the BeadChip staining ra

Pagina 15 - Wash BC2

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 22 of 22[ ] 4. In the Settings area, click E

Pagina 16

Infinium® II Assay HC Two-Sample, Automated (Pre-Amp)Experienced User CardPart # 11280811 Rev. A Page 3 of 22[ ] 4. Vortex each WG#-DNA plate:• For

Pagina 17

Infinium® II Assay HC Two-Sample, Automated (Pre-Amp)Experienced User CardPart # 11280811 Rev. A Page 4 of 22Estimated TimeRobot time: 5 minutes per

Pagina 18 - XStain BC2

Infinium® II Assay HC Two-Sample, Automated (Pre-Amp)Experienced User CardPart # 11280811 Rev. A Page 5 of 22Estimated TimeRobot time:• 20 minutes pe

Pagina 19

Infinium® II Assay HC Two-Sample, Automated (Pre-Amp)Experienced User CardPart # 11280811 Rev. A Page 6 of 22[ ] 4. (Non-Infinium LIMS only) Make s

Pagina 20

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 7 of 22Estimated TimeRobot time:• 10 minutes f

Pagina 21 - Image BC2

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 8 of 22[ ] 2. Click OK in the message box. R

Pagina 22

Infinium® II Assay HC Two-Sample, Automated (Post-Amp)Experienced User CardPart # 11280811 Rev. A Page 9 of 22[ ] 3. Centrifuge the sealed AMP2 pla

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